Domain Editor-in-Chief: Jody W. Deming; Department of Biological Oceanography, University of Washington, Seattle, Washington, United States
Associate Editor: Laurenz Thomsen; Department of Earth and Space Sciences, Jacobs University Bremen, Germany, Bremen, Germany


Introduction

In the changing Arctic, predictions of organic carbon and nitrogen fluxes in marine food-webs are critical to understanding future ecosystem productivity and the efficiency of energy transfer to higher trophic levels (Leu et al., 2011; Tamelander et al., 2013). Concentrations and ratios of particulate organic carbon (POC) and nitrogen (PON) in both sea ice and the water column are important parameters for Arctic ecosystem modelling at a time when patterns of Arctic productivity and the transitional period from ice algae to phytoplankton are changing (Arrigo, 2013). Biogeochemical modelling (e.g., process and regional models), using appropriate measured parameters, is an important tool for the integration of Arctic climate and ecological system variability, enabling the description and prediction of current and future states of Arctic ecosystems (Tedesco et al., 2012).

The fundamental relationship between carbon and nitrogen ratios (carbon:nitrogen, C:N) sets the stoichiometric basis for modelling biogeochemical processes in aquatic systems. The classical Redfield ratio (carbon106:nitrogen16:phosporus1; Redfield et al., 1963) provided the theoretical and stoichiometric foundation for elemental relationships between particulate and dissolved components of marine and freshwater systems. Although the Redfield ratio is widely used in modelling and nutrient-based production estimates (Mills and Arrigo, 2010; Sambrotto, 2014), departures from the classical Redfield proportions and plasticity in C:N ratios are well-documented for both particulate and dissolved fractions (Sterner et al., 2008; Martiny et al., 2013; Letscher and Moore, 2015). Variability in the elemental ratios of seston has been linked to several factors including inter-species differences, storage products and impacts of varying nutrient conditions (Geider and La Roche, 2002; Arrigo, 2005; Frigstad et al., 2011).

Reliance on the assumption of a fixed C:N ratio may limit or misdirect the modelling of biogeochemical cycles in large or small scale models (Vancoppenolle et al., 2013). In this regard, recent reviews have considered specificity and scale-dependence of stoichiometric ratios (e.g., C:N) for marine and freshwater ecosystems (Sterner et al., 2008) as well as for waters of the Arctic Ocean and pan-Arctic shelves (Frigstad et al., 2014). These recent reviews propose that the classical Redfield model be reassessed to better reflect regionally meaningful relationships for POC:PON ratios. Improved parameterization of seston POC:PON ratios is therefore required to enhance the modelling of carbon cycling including autotrophic production estimates, air-sea CO2 flux and carbon sequestration.

Similar to water column modelling, sea-ice models have also relied on POC:PON ratios for the parameterization of ice-associated biogeochemical cycles (Lavoie et al., 2009; Deal et al., 2011; Tedesco et al., 2012). However, local and regional descriptions of sea-ice stoichiometry are largely lacking for POC, PON, dissolved organic carbon and dissolved nitrogen components. Ice-associated autotrophic production contributes seasonally significant amounts of organic biomass during the bloom period (Riedel et al., 2008; Gradinger, 2009; Różańska et al., 2009), and directly contributes to organic carbon fluxes impacting biochemical processes in both the water column (Michel et al., 2002; Pineault et al., 2013; Yamamoto et al., 2014) and sediments (Renaud et al., 2007). The objective of this paper is to provide an overview of POC:PON ratios in first-year sea ice on Canadian Arctic shelves, investigating both seasonal and regional variability. Data coverage includes the productive spring period as well as the dark winter period. Regional comparisons are made between first-year sea ice on the Beaufort Sea shelf (BSS) and first-year sea ice from a productive region of the Canadian Arctic Archipelago (CAA). We hypothesized that sea-ice POC:PON ratios will decrease with increasing sea-ice biomass, as reported for seston stoichiometric ratios in temperate and Arctic waters (Sterner et al., 2008; Frigstad et al., 2014).

Methods

First-year sea ice was sampled in two distinct areas of the Canadian Arctic between 2001 and 2013. In the Canadian Arctic Archipelago (CAA), spring to early summer sampling was conducted around Cornwallis Island during first-year landfast seasonal studies (2001–2003) based from ice camps in southern McDougall Sound. Spatial sea-ice studies were conducted between 2010 and 2013 with 78 stations, primarily pack ice, sampled in Barrow Strait, Resolute Passage, McDougall Sound and Wellington Channel. On the interior Beaufort Sea shelf (BSS) a seasonal sea-ice study (Canadian Arctic Shelf Exchange Study, CASES) was conducted in Franklin Bay between February and June 2004. A spatial survey of winter sea ice was also conducted in the Amundsen Gulf as part of the Circumpolar Flaw Lead (CFL) system study in January 2008. The locations of the sampling stations are shown in Figure 1 and the sampling programs are summarized in Table 1.

doi: 10.12952/journal.elementa.000078.f001.
Figure 1.  

Map showing the location of the sampling stations (A) and the distribution of spring (green), dark winter (blue) and seasonal (red) stations (B).

BS: Barrow Strait; BSS: Beaufort Sea; CAA: Canadian Arctic Archipelago; FB: Franklin Bay; MS: MacDougall Sound; WC: Wellington Channel.

10.12952/journal.elementa.000078.t001

Table 1.

Summary of sampling programs from the Canadian Arctic Archipelago (CAA) and Beaufort Sea shelf (BSS)a

Arctic Shelf Season Sampling dates Study year(s) Study type Ice type n POC:PON (mol:mol)
Average Range
CAA Spring-Summer 29 April–1 July 2001–2003 Seasonal Landfast 100 8.3 4.8–15
CAA Spring 1–18 May 2010–2013 Spatial Landfast, pack ice 78 9.8 6.4–17
BSS Spring 24 Feb–20 June 2004 Seasonal Landfast 44 7.8 3.0–24
BSS Winter 7–27 January 2008 Spatial Pack ice 35 23 12–46

In total, 257 samples of bottom sea ice were analyzed. The bottom 3-cm section of the ice core was routinely retained, targeting the expected majority of the biomass in first-year sea ice of the BSS and CAA. At each station basic sea-ice thickness and snow depth measurements were taken. All sea-ice samples for the determination of chlorophyll a (chl a), particulate organic carbon (POC) and nitrogen (PON) were collected with a manual ice corer (Mark II coring system, 9-cm internal diameter, Kovacs Enterprise). On each sampling day, three to five bottom core sections were collected, pooled together, and melted in the dark with the addition of filtered (0.2 µm) surface water collected at the time of sampling (Garrison and Buck, 1986). Particulate concentrations were corrected for dilution by the added surface water.

Chlorophyll a subsamples were filtered onto 25-mm Whatman GF/F filters and extracted for 24 h in 90% acetone at 4°C in the dark, for fluorometric determination (10AU Turner Designs), according to Parsons et al. (1984). POC and PON were analyzed from the same pre-combusted 21-mm GF/F filter, without prior acidification. Duplicate filters were either dried at 60°C and stored in sealed sterile Petri dishes at room temperature or frozen at −80°C until analysis on a Perkin Elmer elemental analyzer. Blank filters were also stored, as above, on each sampling day; average blank values were subtracted from measured sample values. A minimum weight of 20 µg of N per filter was targeted to ensure POC and PON values were well above detection limits. POC:PON is presented as molar ratios unless otherwise stated.

Inorganic carbon contributions were likely negligible in the study areas, as there was no evidence of shells or terrestrial material that would have contributed particulate inorganic carbon to the sea ice (e.g., the Prymnesiophycaea spp. reported in Riedel et al., 2003, and Różańska et al., 2009, were not coccolithophores), and the methods used here are inadequate to retain ikaite crystals (Dieckmann et al., 2010). Inorganic nitrogen contributions were also likely negligible, as the presence of nitrogen bound to inorganic particles (e.g., ammonium-bound clay minerals) or nitrogen adsorbed to particles has not been documented in Arctic sea ice to our knowledge. To test these assumptions, additional POC/PON samples were acidified during the 2004 BSS and 2011 CAA sampling campaigns (n = 33). The acidified POC and PON samples made up, on average, 93% (± 12%, range of 59–118%) and 98% (± 20%, range of 60–161%) of the values of non-acidified samples, respectively (for internal consistency, only the non-acidified values have been included in this study). Therefore the POC and PON values presented herein are considered reasonable estimates of the particulate organic fraction within the sea ice of both shelves.

Statistical analyses

To test the hypothesis that POC:PON ratios change with varying concentrations of sea-ice biomass, Model II (standardized major axis [SMA], 95% confidence intervals) linear regressions of log-transformed data were estimated with R statistical software. Model II linear regressions (Sokal and Rohlf, 1995) were used, as both POC and PON are affected by biological sources of stochasticity and analytical error. A linear relationship between POC and PON assumes that the POC:PON ratio remains constant over the range of POC and PON measurements (e.g., POC = 6.6*PON for Redfield). This assumption was tested by determining if the slopes of the Model II regressions were significantly different than 1 in log-log space (T-test, α = 0.05).

If POC:PON ratios are found to vary, a power function relationship following the arguments of Sterner et al. (2008) and Frigstad et al. (2014), could be used to describe the POC:PON ratios over the range of observed POC and PON concentrations, as in equations 1-3.

POC = aPONb
(1)

or

log(POC) = log(a) + b  log(PON)
(2)

or

POC: PON = aPON(b1)
(3)

where:

POC is the concentration of POC (µmol L−1), PON is the concentration of PON (µmol L−1), a is a proportionality constant with log(a) corresponding to the y-intercept at PON = 1 µmol L−1, and b is the slope of the regression. A slope (b), if significantly different than 1, indicates that the POC:PON ratio varies over the range of measurements. When b < 1, the POC:PON ratio decreases with increasing POC and PON concentrations; when b > 1, the POC:PON ratio increases with increasing POC and PON concentrations.

To identify relationships between POC:PON ratios and selected measured variables (e.g., ice thickness), a standard linear model was used since the significance of the relationship cannot be tested with a SMA model regression. To test for significant differences in measured POC:PON ratios between the CAA and BSS regions, a non-parametric Mann-Whitney test was used on untransformed data. A Wilcoxon Signed Rank test was also used to compare measured POC:PON ratios with the Redfield ratio. Statistical tests were performed with JMP (SAS).

Results

Sea-ice thickness averaged 157 cm (range of 77–214 cm, n = 224) during the spring sampling period and 75 cm (range of 1–112 cm, n = 35) in January during the dark winter. Measured snow depths averaged 11.8 cm (range of 1.1–42 cm, n = 224) and 4.2 cm (range of 0–12 cm, n = 35) in the spring and dark winter, respectively.

Chlorophyll-based sea-ice biomass

Autotrophic sea-ice biomass was consistently concentrated at the ice-water interface of all ice cores. For example, in full cores collected in the CAA in 2011 and 2013 over 97% of the chl a biomass was located in the bottom 3 cm of the entire ice core. Therefore the POC:PON ratios presented herein are representative of the bulk of the autotrophic particulate organic matter within ice cores of the study regions. However, POC:PON ratios should not be considered uniform throughout the ice thickness. The ratios presented here are not readily applicable to upper ice sections of first-year ice cores, nor multi-year sea ice. In addition bottom ice concentrations of particulate material, including chl a and POC, should not be integrated over the entire thickness of the ice as this would grossly overestimate total sea-ice biomass concentrations.

In the productive CAA, chl a concentrations in bottom first-year sea ice were on average 4.4 times higher than concentrations in the BSS during spring (Figure 2). Maximum chl a concentrations occurred during May on both shelves with generally gradual declines to concentrations around or below 100 µg L−1 by the end of June. During the spring period which included the onset of growth and peak biomass (i.e., bloom) of ice algae, chl a concentrations were on average 1022 and 233 µg L−1 in the CAA and BSS, respectively, with maximum values reaching 4560 µg L−1 in the CAA and 870 µg L−1 in the BSS. In January during the dark winter, chl a concentrations averaged 0.08 µg L−1 with a maximum of only 0.19 µg L−1. This study provides a unique dataset covering sea-ice chl a biomass spanning four orders of magnitude.

doi: 10.12952/journal.elementa.000078.f002.
Figure 2.  

Temporal trends in POC:PON ratios and chl a concentrations in bottom first-year sea ice during spring and early summer.

POC:PON ratios and chlorophyll a (chl a) concentrations during spring-early summer seasonal studies from the Canadian Arctic Archipelago (CAA) and Beaufort Sea shelf (BSS) are plotted by sampling date. Ice cores during seasonal studies were collected where snow depths were less than 20 cm.

POC:PON ratios

Average and range of POC:PON ratios for measured POC and PON concentrations are summarized in Table 1 for the CAA and BSS shelves during the spring and dark winter periods. The POC:PON ratios on the BSS shelf during spring displayed higher variability (Figure 3a) and were significantly lower (Mann-Whitney, p < 0.01) than spring ratios for the CAA (Table 1). POC:PON ratios generally increased during the spring period and tended to increase or remain stable in association with the onset of the melt period (Figure 2). Monthly variations in POC:PON ratios were evident for both the CAA and BSS. January POC:PON ratios displayed a large range of variability and were significantly higher (Mann-Whitney, p < 0.01) than spring ratios (Table 1, Figure 3a). Spatial variability in POC:PON ratios was best displayed by the CAA spatial surveys conducted between 2010 and 2013 (Table 1). Figure 4 shows the POC:PON distribution for the CAA region surrounding Cornwallis Island. The lowest ratio (6.1) was observed at the western end of the Barrow Strait sampling area with the nearshore region of Resolute Passage also showing consistently low ratios. High ratios (14–17) were measured at the northern extent of the sampling region in both McDougall Sound and Wellington Channel.

POC:chl a ratios (g:g) showed similar monthly trends to those observed for POC:PON ratios (Figure 3). During the spring period, POC:chl a ratios ranged between 17 and 560 (average 81) and 10 and 870 (average 52) in the BSS and CAA, respectively. The ratios were not significantly different between the BSS and CAA study regions (Mann-Whitney, p = 0.06). There were a few very high POC:chl a ratios (> 1000) at the beginning (e.g., February 24th) of the growth period in the BSS, but similar high ratios were observed at the end of the sampling period (e.g., June 29th) in the CAA (Figure 3a). The very low chl a content of the dark winter sea ice was evident from the extremely high POC:chl a ratios (average 8400, range 1478–23800) in January.

doi: 10.12952/journal.elementa.000078.f003.
Figure 3.  

Monthly variation from winter to spring in bottom sea-ice POC:PON and POC:chl a ratios.

Ratios of POC:PON (A) and POC:chl a (B) from the Canadian Arctic Archipelago (CAA) and Beaufort Sea shelf (BSS) are plotted by sampling month. CAA data (black) have been offset from BSS data (red) for better visibility.

doi: 10.12952/journal.elementa.000078.f004.
Figure 4.  

Spatial variability of bottom sea-ice POC:PON ratios in the Canadian Arctic Archipelago during spring.

Color scale shows spatial variability of POC:PON ratios (mol:mol) in the bottom sea ice of Barrow Strait (BS), Resolute Passage (RP), McDougall Sound (MS) and Wellington Channel (WC) during May 2010–2013.

Combining the dark winter and spring periods resulted in a weak but significant seasonal decrease in POC:PON ratios with increasing chl a concentrations (log-transformed, r2 = 0.24, p < 0.01). When considering only the spring period, a weak but significant increase in POC:PON ratios occurred with increasing chl a concentrations (log-transformed, r2 = 0.11, p < 0.01). There was no significant relationship between POC:PON ratios and ice thickness; however, POC:PON ratios did show a weak significant decreasing trend with increasing snow thickness (r2 = 0.12, p < 0.01). When compared to the Redfield ratio, the measured POC:PON ratios were significantly higher for the dark winter period (Wilcoxon, p < 0.01) and for the spring period in the CAA alone and the CAA and BSS measurements combined (Wilcoxon, p < 0.01). The spring sea-ice POC:PON ratios from the BSS were not significantly different than the Redfield ratio (Wilcoxon, p = 0.1; Table 1).

Model II linear regressions between log-transformed POC and PON values for the spring and dark winter are presented in Figure 5. The log-transformed regression lines were log(POC) = 0.634 + 1.12(log[PON]) (r2 = 0.94) and log(POC) = 1.39 + 0.793(log[PON]) (r2 = 0.66) for the spring (CAA and BSS combined) and dark winter (January) data, respectively. The SMA slopes for the spring (CAA and BSS combined and separate) and dark winter data were all significantly different than 1 (p < 0.05). The anti-log of the y-intercept estimates the POC:PON ratio when PON is equal to 1 µmol L−1. A summary of POC:PON ratios from the Model II SMA regression, scaled to average PON concentrations for each study period, is provided in Table 2. The POC:PON ranges in Table 2 were calculated from the 95% confidence intervals of the SMA regressions.

doi: 10.12952/journal.elementa.000078.f005.
Figure 5.  

POC versus PON concentrations in bottom sea ice during the dark winter and spring periods.

Log-log SMA regressions of POC versus PON concentrations (µmol L–1) in bottom first-year sea ice during the dark winter (blue line) and spring (black line) periods. Spring regression combines concentrations from both the Canadian Arctic Archipelago (CAA) and Beaufort Sea shelf (BSS). Dashed green line indicates the classical Redfield ratio.

10.12952/journal.elementa.000078.t002

Table 2.

Summary of first-year sea-ice POC:PON ratios and their 95% confidence interval (CI) range, calculated from Model II SMA regressionsa

Arctic Shelf Season POC:PON (mol:mol) CI 2.5% CI 97.5% r2
CAA Spring 12 8.4 19 0.93
BSS Spring 6.7 4.4 13 0.88
CAA + BSS Spring 9.8 6.3 15 0.94
BSS Winter 29 7.6 56 0.66

Given that the POC:PON ratios changed with varying concentrations of sea-ice biomass (i.e., SMA slopes significantly different than 1, p < 0.05), a power function (equation 1) could be used to describe the relationship between sea-ice POC and PON concentrations. Power function equations derived to estimate POC:PON ratios were POC:PON = 4.31 PON0.12 and POC:PON = 24 PON−0.21 for the spring (CAA and BSS combined) and dark winter (January) periods, respectively. In Figure 6, POC:PON ratios estimated using the power function equations are shown with the range of PON concentrations measured in this study.

doi: 10.12952/journal.elementa.000078.f006.
Figure 6.  

POC:PON power function relationships over the measured range of bottom first-year sea-ice PON concentrations.

Relationships are shown in log-log space for first-year sea-ice POC:PON ratios over the measured range of sea-ice PON concentrations for the dark winter (blue line) and spring (red line). The shift in POC:PON relationship between dark winter and spring periods is denoted by vertical hatched line. The average and standard deviation of sea-ice POC:PON ratios are plotted for PON concentration groupings, as follows: ratios < 1, 1–2, > 2–6, > 6–50, > 50–100, > 100–300, > 300–600 and > 600 µmol L–1. The sample size (n) is indicated for each group.

Discussion

First-year sea ice on Canadian Arctic shelves is characterized by highly concentrated biomass at the ice-water interface. Microorganisms and protists begin colonizing the ice at the time of ice formation (Riedel et al., 2007; Różańska et al., 2008) and autotrophic production can commence early in the spring (e.g., in March; Riedel et al., 2006) when light intensities are sufficient (Różańska et al., 2009). With the onset of autotrophic production there is a rapid shift in species composition with heterotrophic groups becoming overshadowed by the biomass of autotrophic species, primarily pennate diatoms during the algal bloom in first-year sea ice of the study regions (Riedel et al., 2008; Różańska et al., 2009; Niemi et al., 2011; Piwosz et al., 2013). The dynamics of growth, decline and loss of the sea-ice community are complex. The availability of light and inorganic nutrients and physical-chemical interactions between the atmosphere, ice and ocean can have community level impacts (Frigstad et al., 2011; Martiny et al., 2013) potentially altering the stoichiometric balance of sea-ice particulate organic matter.

Sea-ice POC:PON

Despite the ecological likelihood of stoichiometric plasticity for carbon and nitrogen ratios, the fixed Redfield ratio (6.6 for C:N; Redfield et al., 1963) continues to be used for parameterization in sea-ice modelling studies (e.g., Lavoie et al., 2009). In this study, average POC:PON ratios for first-year ice on Canadian Arctic shelves during the spring were generally higher than the Redfield ratio (Tables 1 and 2), similar to POC:PON ratios from landfast ice in the Chukchi Sea (8.9 mol:mol; Jin et al., 2006) as well as in Antarctic sea ice (9.4 mol:mol; Fransson et al., 2011). The POC:PON ratios for the first-year sea ice in spring were generally indicative of a growing, non-degraded autotrophic community (Pineault et al., 2013).

The spring POC:PON ratios from the BSS were not significantly different (p = 0.1) than the Redfield ratio whereas the POC:PON ratios from the CAA were significantly higher (p < 0.01), indicating that on the more productive CAA shelf (Michel et al., 2006, 2015), sea-ice particulate organic matter would be more carbon-rich, or slightly nitrogen-depleted, than is assumed based on the classical Redfield proportions. This carbon to nitrogen imbalance has consequences for nitrogen-based models; for example, for estimates of production based on nutrient drawdown. For bottom sea ice in the CAA, using Redfield instead of the average regression POC:PON ratio from the CAA in this study (12 for the CAA; Table 2) would result in an underestimation of organic carbon production by a factor of 1.8. Upward scaling, to represent regional estimates would carry this factor, suggesting that underestimates of production by a factor of two are not unlikely based on POC:PON ratios alone. For the spring BSS and CAA values combined, the relationship between POC and PON follows the Redfield ratio (Figure 5), and the 95% confidence interval (6.3–15; Table 2) does overlap with the Redfield ratio in the lower range of observations, suggesting that the Redfield ratio could provide a reasonable approximation of spring sea-ice POC:PON. However, the combined spring POC:PON ratios were significantly higher than the Redfield ratio (p < 0.01). Incidentally, estimating sea-ice PON concentrations for which the Redfield ratio applies using the SMA spring regression generates PON concentrations between 22.9 and 52.8 µmol L−1 (logPON 1.4–1.7) for a 5% confidence interval on Redfield values, and between 14.6 and 77.8 µmol L−1 (logPON 1.2–1.9) for a 10% confidence interval. These PON concentrations respectively represent only 5.8% and 12% of spring observations (Figure 5), thereby demonstrating the need to use variable POC:PON ratios to provide a realistic representation of the actual range of observations in first-year sea ice. Therefore, the selection of a single ratio to represent sea-ice POC:PON requires careful consideration especially in highly productive areas (e.g., CAA) and throughout an annual cycle that includes a wide range of sea-ice POC and PON concentrations.

Figure 5 clearly shows that the Redfield ratio is not applicable to bottom sea-ice POC:PON during the dark winter period (Figure 5, Table 2). During the dark winter period, bacteria and both autotrophic and heterotrophic protists continue to be incorporated into the growing sea ice (Niemi et al., 2011) and nil or very low light levels preclude photosynthetically-derived autotrophic production. POC is largely decoupled from chl a concentrations during the dark period (Figure 3b) with significant contribution of organic carbon coming from non-pigmented or low-pigmented biomass in a system that is driven by heterotrophic/mixotrophic production. Sea-ice POC:PON ratios during that time decrease with increasing biomass, opposite the spring trend (Figure 5).

POC:PON ratios in the bottom sea ice during spring were similar to average seston POC:PON from temperate (8.3; Sterner et al., 2008) and Arctic (7.4; Frigstad et al., 2014) waters. The variability of spring sea-ice POC:PON in this study (Figure 3a) was also comparable to the range of variability for seston POC:PON ratios in the Arctic Ocean, Chukchi Sea and polynya areas (e.g., the North Water; Frigstad et al., 2014). Future detailed comparisons between sea-ice and under-ice seston POC:PON ratios would be useful for organic carbon and nitrogen flux estimates during the spring and especially the melt period.

Temporal variability

Temporal variability in POC:PON ratios was evident at a seasonal (spring; Figure 2) and monthly (January–July; Figure 3a) scale. The general increase in bottom ice POC:PON ratios during the spring period in both the CAA and BSS (Figure 2) is consistent with observations of higher POC:PON ratios in phytoplankton toward the end of the productive period in the North Water polynya (Mei et al., 2005). Seasonal increases in POC:PON ratios have been attributed to carbon overconsumption later in the season when nutrients are drawn down or depleted. Earlier in the spring, ratios can be lower due to the production of nitrogen-rich organic material in the absence of nutrient limitation (Bates et al., 2005; Mei et al., 2005). With trends towards a shorter Arctic ice season and earlier melt, sea-ice biomass may be released prior to the onset of seasonal conditions that elevate sea-ice POC:PON ratios. Consequently, the organic carbon contribution of sea-ice fluxes during melt would be reduced relative to nitrogen, altering carbon budgets for predictive ice-ocean coupled biogeochemical models.

High variability in POC:PON ratios persisted for each month regardless of whether sea-ice biomass was low (February–March) or at its peak (May–June). The range of monthly variability was also very high for POC:chl a (Figure 3b) ratios. The high POC:chl a ratios (> 500) from the BSS sea ice in February and March were likely due to the numerical dominance of non-autotrophic species in the sea-ice community (Riedel et al., 2008; Różańska et al., 2009; Frigstad et al., 2011). In contrast, the high POC:chl a measured towards the end of the season (e.g., > 2000 in June from CAA sea ice) may have been driven by the production of carbon-rich particulate exopolymers that can be retained within the sea ice even when chl a biomass is lost during melt (Riedel et al., 2008; Juhl et al., 2011). These findings suggest that drivers of biochemical ratios in first-year sea ice vary seasonally over an annual cycle.

The dark winter period is clearly distinct with respect to elemental concentrations and ratios in the sea ice. The transition from the dark period to the spring growth period in first-year sea ice represents a drastic stoichiometric shift in POC:PON ratios (Figure 6) occurring with the early onset and increase in irradiance. Dark period sea-ice POC and PON were collected in the Amundsen Gulf where terrigenous organic carbon from Mackenzie River waters could impact stoichiometric ratios in the ice and water. However, during the winter period river water is largely contained near shore, away from the dark winter sampling area, by the formation of the stamukhi ice field (Carmack et al., 2002). The dark winter bottom ice community is comprised of a similar number of taxa as the spring community (Niemi et al., 2011) indicating that the diversity of the sea-ice community is largely established as cells are incorporated into the ice prior to the onset of the ice algal growth period. Therefore, models which attempt to capture baseline conditions for first-year sea-ice communities should reflect the, on average, order of magnitude decline in POC:PON ratios (i.e., from 29 to 9.8; Table 2) that occurs with the transition from the dark to light period.

Spatial variability

Spatial variability in POC:PON ratios was evident at local (Figure 4) and regional scales (i.e., BSS versus CAA; Tables 1 and 2). In the productive CAA, POC:PON ratios varied 2.7 fold within the vicinity of Cornwallis Island during a limited time period (early May; Table 1, Figure 4) covering the ice algal bloom (Figure 2; Smith et al., 1993; Michel et al., 2006). At the larger regional scale, POC:PON ratios in first-year sea ice were significantly lower on the BSS as compared to the CAA (Table 2). From an ecological perspective, the higher nitrogenous content per unit of carbon biomass in the BSS sea ice may benefit the growth of herbivorous grazers (Malzahn et al., 2010; Frigstad et al., 2011) that consume ice algae when phytoplankton biomass is low under ice-covered conditions.

The observed regional variability with higher POC:PON ratios in the CAA is consistent with the weak significant positive relationship between bottom ice POC:PON ratios and chl a concentrations during spring. The drivers of spatial variability in seston or sea-ice POC:PON ratios at local or regional scales are not directly evident, and their analysis is beyond the scope of this paper. Chlorophyll a is a proxy that integrates multiple processes that could alter POC:PON ratios over a range of spatial and temporal scales. The observed relationship between sea-ice POC:PON ratios and chl a concentrations implies that several factors could be impacting the POC:PON ratios, including bloom dynamics or stages of algal growth, cell viability and physiology, nutrient availability and light acclimation responses (Frigstad et al., 2011; Ayata et al., 2014; Talmy et al., 2014). Snow cover was also weakly negatively related to first-year ice POC:PON ratios indicating that factors altering irradiance at the bottom of the ice as well as ice growth/melt processes (Campbell et al., 2015) may also influence stoichiometric relationships within the bottom ice. Detailed analyses of sea-ice and surface water variables and further process studies are required to identify which variable or combination of variables determine stoichiometric relationships within first-year sea ice.

Biomass-Ratio relationships

Seston POC:PON ratios are reported to decrease with increasing seston concentrations (Sterner et al., 2008; Frigstad et al., 2014). Similar trends have also been observed for exported organic material (Tamelander et al., 2013). For the linear relationships between POC and PON (Figure 5) a log-log SMA slope < 1 implies that POC:PON ratios decrease with increasing concentrations of POC and PON. Based on the previous observations of wide-scale trends for water column POC:PON, we originally hypothesized that sea-ice POC:PON ratios would also decrease with increasing sea-ice biomass. This hypothesis could not be validated for first-year sea ice during spring, as the slopes were significantly higher than 1 and POC:PON ratios significantly increased with increasing PON concentrations (Figure 6). Both temporal (Figure 2) and spatial (Figure 5, Table 2) increases in POC:PON ratios with increasing biomass in spring contribute to the rejection of the working hypothesis for the productive period. The hypothesized relationship between POC:PON ratios and sea-ice biomass was only upheld during the dark winter period when POC:PON ratios decreased with increasing PON concentrations (Figure 6), or when considering the full seasonal range from the dark winter to spring.

The decrease in POC:PON ratios at increasing seston concentrations has been linked to shifting nutrient use efficiencies (e.g., physiological status of autotrophs; Sterner and Elser, 2002) as well as shift in seston composition (Frigstad et al., 2011). Autotrophic material, with a generally lower POC:PON than non-autotrophic protists (Frigstad et al., 2011), would drive the seston POC:PON ratio downwards as chlorophyll biomass increases and autotrophic material comprises a greater proportion of the seston. A similar increase in autotrophic material, with respect to both biomass and abundance, occurs in bottom first-year sea ice during the growth period (Michel et al., 2003; Riedel et al., 2008), yet the sea-ice POC:PON ratios do not appear to decline accordingly (Figures 2 and 3a). Therefore, the proportional composition of the sea-ice community and the presence of key ice-algal species (i.e., diatoms) do not appear to be the strongest drivers of biomass-ratio stoichiometric relationships within the bottom ice. Complex biological (e.g., shift in species composition, as in Michel et al., 1996; Riedel et al., 2008) and physiological (e.g., pigment composition, as in Mundy et al., 2011; protein/lipid cellular allocation, as in Cota and Smith, 1991; Mock and Gradinger, 2000) interactions, together with environmental forcings that influence nutrient availability and uptake (e.g., nitrification, as in Fripiat et al., 2014; nutrient status, as in Granskog et al., 2003) are to be considered to explain POC:PON ratios in multifaceted sea-ice communities. In addition, the relative importance of environmental forcings for POC:PON ratios will likely shift under conditions of Arctic change, thereby impacting predictive modelling of sea-ice biogeochemical cycling. For example, the relative influence of nutrient availability on bottom ice POC:PON ratios may shift from external (e.g., nutrient concentrations at the ice-water interface) to internal (e.g., sea-ice remineralisation processes) drivers as sea-ice melt and other environmental forcings (e.g., stratification, Arrigo, 2013; Tremblay et al., 2015) alter nutrient cycling in Arctic surface waters.

Bottom sea-ice differs from the pelagic environment in that it contains a highly concentrated layer of biomass, similar to that of a biofilm, rich in carbon and abundant in extracellular organic carbon (Krembs et al., 2002; Meiners et al., 2003; Riedel et al., 2006; Juhl et al., 2011). Sea-ice exopolymer carbon production is closely linked to ice algae (Underwood et al., 2013) such that the drivers favoring both increased (i.e., carbon-rich polymers) and decreased (i.e., autotrophic material) POC:PON ratios could offset expected biomass-ratio trends. Physiological changes in cell status and changes in community composition are likely to be as important in affecting POC:PON ratios within sea ice as for the water column. However, the distinct habitat and growth conditions in sea ice (e.g., low temperatures, self-shading, melt processes) need to be considered when interpreting POC:PON ratio trends.

Conclusions

Using the Redfield ratio for POC:PON conversion would provide reasonable estimates only over a limited range of sea-ice biomass concentrations observed in this study. Therefore, our results argue in favour of using variable POC:PON stoichiometry in sea-ice biogeochemical models, supported by the wide range of biomass concentrations in first-year sea ice and the evidence of variability in sea-ice POC:PON ratios at the regional (CAA), shelf (CAA versus BSS), and seasonal (dark winter versus spring) scales. Incorporating this variability into analytical and predictive modelling efforts is essential to achieve a better understanding of the role of first-year sea ice in regional food webs and global biogeochemical cycles. The use of the power function model presented here is recommended, as it reflects that POC:PON ratios do not remain constant over the observed sea-ice POC and PON concentrations. For sea-ice biogeochemical modellers, recommendations include: 1) parameterization using variable POC:PON ratios rather than consistent Redfield or average values, 2) the inclusion of distinct power functions to parameterize dark winter versus spring POC:PON ratios and, 3) Arctic-wide or regionally-based models based on areal (i.e., m2) estimates should not apply bottom ice stoichiometry to the upper sections of first-year sea ice.

The distinct dynamics of POC:PON ratios during the dark winter and spring periods (Figure 6) clearly show that spring parameters should not be extrapolated to winter, for annual budget estimates or simulations. Surprisingly, our original hypothesis that POC:PON ratios would decrease with increasing biomass, as observed in Arctic waters (Frigstad et al., 2014), was valid only during the dark winter period. Further to these findings, we hypothesize that the relationship between POC:PON ratios and bottom sea-ice biomass during the dark winter period is applicable to stoichiometric relationships within upper ice sections, including in multi-year sea ice, and possibly in first-year sea ice replacing multi-year ice in new areas of the high Arctic where ice biomass may remain low due to light limitation.

Data accessibility statement

All data will be publically available from BioChem: database of biological and chemical oceanographic data. Fisheries and Oceans, Canada. http://www.meds-sdmm.dfo-mpo.gc.ca/biochem/biochem-eng.htm.

Copyright

© 2015 Niemi and Michel. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Contributions

Contributed to conception and design: AN, CM

Contributed to acquisition of data: AN, CM

Contributed to analysis and interpretation of data: AN, CM

Drafted and/or revised the article: AN, CM

Approved the submitted version for publication: AN, CM

Competing interests

The authors do not have any competing interests.

Funding information

NSERC (Natural Sciences and Engineering Research Council of Canada) Research Network Grant, and Discovery Grants to CMDFO Strategic Research Fund, International Governance Strategy and NCAARE (National Centre for Arctic Aquatic Research Excellence) to CM Canada Foundation for Innovation and Fonds québécois de la recherche sur la nature et les technologies (FQRNT) OERD (Office of Energy Research and Development), NRCan (Natural Resources Canada) to CM PCSP (Polar Continental Shelf Program) logistical and in-kind support NSTP (Northern Science Training Program).