Information integral to a full understanding of the article but that is in formats that (a) cannot be rendered in two dimensions or (b) are too large to be clearly represented in current viewing systems (Web browsers, e-readers, PDF) should be submitted as Supplemental Material. Examples of this category of supplemental material include very large tables, audios, videos, three-dimensional visualizations, interactive graphics, and so on.
In addition, content providing relevant information that meaningfully enhances the article but is not critical to its assertions or conclusions may be submitted as supplemental material. These materials can take the form of additional figures and tables, as well as rich media. We encourage authors to always consider clarity and ease of use for readers when presenting supplemental material: consider whether some supplementary files work best as a composite file (e.g. text, table, and figure in one downloadable document file – see sample here) or whether they are most useful downloaded separately as individual files. Please note, Editors are free to request changes to how supplemental files are presented to ensure clarity for readers.
Note: All supplemental material should be submitted along with your manuscript for review. Supplemental material files are published as supplied including all text formatting, figure sizing and placement.
Although we do not limit the number or type of Supplemental Material items authors may include, we do require that they provide a relevant and useful expansion of the article, and that they be as well described as are figures and tables included within the body of the article. Good metadata of this material are key to discoverability and usefulness. All Supplemental Material should include the following:
Supplemental material must be cited at the appropriate point within the main text, just as main text figures and tables. This offers readers context and allows for seamless interlinking. Text citations should use the appropriate type and number designation (e.g., Video S1).
Figures should be placed in the composite supplemental material file (preferably PDF file). Please see our Figure preparation section for detailed figure preparation instructions.
Tables should be placed in the composite supplemental material file (preferably PDF file). Please see our Table preparation section for detailed table preparation instructions.
For videos, please try to submit videos of reasonable quality. We highly recommend videos to be submitted in MP4 format. Whatever format you use, videos must open and play in either QuickTime Player v. 7.6.2 or Windows Media Player v. 11.
We prefer that Supplemental Material files not exceed 10MB. However, if that size limit results in a loss of quality (e.g., by making the dimensions smaller or compressing a movie in such a way as to compromise image quality), we can accept larger files.
We require that all datasets associated with an article be made openly available, unless there are clear reasons to restrict access. Datasets should be archived and published in any stable domain or general repository.
We require that authors include a “data accessibility statement” (DAS) with their submission. This should list the database(s) and the respective accession numbers and DOIs for all data from the manuscript that has been made publicly available. For example:
-DNA sequences: Genbank accessions F234391-F234402; NCBI SRA: SRX0110215
-Final DNA sequence assembly: uploaded as online supporting information
-Phylogenetic data: TreeBASE Study accession no. S9345
-R scripts: uploaded as online supporting information
-Sample locations, IMa2 input files and microsatellite data: DRYAD entry doi:10.5521/dryad.1311
If applicable, a data accessibility statement must be present in your manuscript at initial submission, and, if the data is not publicly available yet should list where each dataset will be archived. There is no requirement to include accession numbers or DOIs at initial submission, but manuscripts lacking a DAS will not be passed through to an Editor-in-Chief.